Composite
Part:BBa_K3002218:Design
Designed by: Dorothée Klein Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 Hygromycin resistance + L1_connector_Pos2 + ccA_MHETase_SP20HA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3126
Illegal PstI site found at 3450
Illegal PstI site found at 3793
Illegal PstI site found at 4603 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2048
Illegal PstI site found at 3126
Illegal PstI site found at 3450
Illegal PstI site found at 3793
Illegal PstI site found at 4603
Illegal NotI site found at 3461 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4371
Illegal BamHI site found at 1408
Illegal XhoI site found at 250 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3126
Illegal PstI site found at 3450
Illegal PstI site found at 3793
Illegal PstI site found at 4603 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3126
Illegal PstI site found at 3450
Illegal PstI site found at 3793
Illegal PstI site found at 4603
Illegal NgoMIV site found at 773
Illegal NgoMIV site found at 955
Illegal NgoMIV site found at 1326
Illegal NgoMIV site found at 3059
Illegal NgoMIV site found at 3520
Illegal NgoMIV site found at 3550
Illegal NgoMIV site found at 3865 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002119 (hygromycin resistance) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).