Composite

Part:BBa_K3002218:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 Hygromycin resistance + L1_connector_Pos2 + ccA_MHETase_SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3126
    Illegal PstI site found at 3450
    Illegal PstI site found at 3793
    Illegal PstI site found at 4603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2048
    Illegal PstI site found at 3126
    Illegal PstI site found at 3450
    Illegal PstI site found at 3793
    Illegal PstI site found at 4603
    Illegal NotI site found at 3461
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4371
    Illegal BamHI site found at 1408
    Illegal XhoI site found at 250
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3126
    Illegal PstI site found at 3450
    Illegal PstI site found at 3793
    Illegal PstI site found at 4603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3126
    Illegal PstI site found at 3450
    Illegal PstI site found at 3793
    Illegal PstI site found at 4603
    Illegal NgoMIV site found at 773
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 3059
    Illegal NgoMIV site found at 3520
    Illegal NgoMIV site found at 3550
    Illegal NgoMIV site found at 3865
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002119 (hygromycin resistance) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).

References